TABLE 4.

Summary of the analysis of TAC1 alleles cloned from clinical isolates and screened by the P_(CDR2)-HIS3 reporter system

Patient	Origin	Strain	Fluconazole MIC (μg/ml)	MTL	Plasmid name	EcoRI profile	AciI profile	Sequence profilec	One-hybrid phenotypee	TAC1 GOF mutation(s)
A	CHUVd	DSY281	1	a/α	1607-1	A			WT
					1607-2	B	WT
		DSY284	32	a/α	1608-8	A	Allele A	HYP	H741Ya
					1608-99	A	Allele B	HYP	G980E + H741Y
B		DSY2014	0.125	a/α	1609-13	A			WT
					1609-12	B	WT
		DSY2015	16	a/α	1621-71b	A	HYP	R673Q
						C		DSY2019	4	α/α	1610-19	A			WT
1610-16	B	HYP	N972D
DSY2020	32	α/α	1622-76	B	HYP			N972D
D		DSY2250	1	a/α	1611-23	A			WT
					1611-24	B	WT
		DSY2251	16	a/α	1477-A	A	WT
					1477-B	B	HYP	N972D
E	CHUV	DSY544	0.5	a/α	1612-30	A			WT
					1612-26	B	WT
		DSY775	128	a/a	1618-56	A	HYP	G980W
F	CHUV	DSY1280	1	a/α	1635-4	A			HYP	E461K
					1617-121	B	HYP	E461K
		DSY1292	16	a/α	1490-A	A	A	HYP	N972S
					1490-C	A	B	HYP	N972S
G	CHUV	DSY2242	8	a/a	1619-61	B			HYP	G980E
		DSY2243	1	a/a	1637-3	B	WT
H	CHUV	DSY555	8	a/α	1620-68	A			WT
					1620-92	B	HYP	R693K
		DSY556	128	α/α	1488-3	A	HYP	R693K
		DSY557	128	α/α	1493-12	B	HYP	R693K
I	CHUV	DSY757	2	a/α	1623-83	A			WT
					1623-82	B	WT
		DSY758	16	a/α	1624-86	B	Allele A	HYP	A736V
					1624-88	B	Allele B	HYP	A736V
J	CHUV	DSY2260	8	a/α	1638-1	A			HYP	E841Ga
					1638-3	B	HYP	E841G
		DSY2262	64	a/α	1613-31	A	A	HYP	E841G
					1639-1	A	B	HYP	E841G
K	CHUV	DSY482	8	a/α	1614-36	B			WT
					1614-138	A	WT
		DSY488	16	α/α	1487-3	A	HYP	A736T
L	CHUV	DSY520	32	a/α	1615-44	A			WT
					1615-41	B	HYP	N972D
		DSY522	128	a/α	1616-46	B	HYP	N972D
					1474-B	A	HYP	N972D
M	CHUV	DSY2305	2	a/α	1633-3	A	A	Allele A	WT
					1633-4	A	A	Allele B	WT
		DSY2306	8	a/a	1634-1	A	From allele A	HYP	E461K
N	CHUV	DSY2023	16	α/α	1528-10	A			HYP	N972D
		DSY2025	16	α/α	1529-2	A	HYP	N972D

^aThis putative GOF mutation was not verified by site-directed mutagenesis.

^bOnly one TAC1 allele was cloned even if the sequence of the genomic DNA indicated a polymorphism at the MTL.

^cTAC1 sequence analysis was performed after failure of TAC1 allele discrimination by RFLP in MTL heterozygous strains.

^dCHUV, University Hospital Lausanne and University Hospital Center.

^eWT, wild type; HYP, hyperactive.