FIG. 10.

Cell death caused by complete depletion of GPATs results in multibudded cells. dko-GAT1 (A) and dko-GAT2 (B) strains were grown in rich medium containing galactose and then shifted to glucose for 12 h. Cells were briefly sonicated in order to separate them, and differential interference contrast (DIC) images were taken in order to analyze their cell morphology. Multibudded cells were detected for both dko-GAT1 and dko-GAT2 cells. Nuclei were visualized with DAPI. The multibudded phenotype was also captured in electron microscopy images of these dead cells. Multiple vesicles surrounding their nuclei were often observed.