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. 2009 Jun 5;191(16):5320–5324. doi: 10.1128/JB.00522-09

FIG. 4.

FIG. 4.

MalT(Con)(W317R) is constitutively active. (A) malT(Con)(T949A) mutants exhibit elevated LamB levels, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane preparations. Cells were grown in LB at 37°C and harvested during late exponential phase. Gels were stained with Coomassie brilliant blue. Lane 1, WT strain (AJW678); lane 2, ompR::Tn10 strain (AJW2050); lane 3, malT(Con)(T949A) strain (AJW3445); lane 4, ompR::Tn10 malT(Con)(T949A) strain (AJW3098). (B) Inhibition of MalT(Con)(W317R) with MalY and Aes. The ompR malT(Con)(T949A) mutant (strain AJW3098) was transformed with plasmids carrying genes coding for MalT inhibitors (MalK, MalY, Aes) or the empty vector. The resulting transformants were streaked on LB plates supplemented with IPTG to induce gene expression, and growth was observed after incubation at 37°C for 48 h.