TABLE 3.
Results of screen for X. nematophila mutants defective in in vitro lipase activity against Tween
| Mutant genotype | XNC1 ORFa | Classb | Predicted protein functionc | E valuec | % Homologyc,d | Homolog organisme |
|---|---|---|---|---|---|---|
| flhD5::Tn10 | 1627 | Regulator | Regulator of flagellar synthesis | 9e−54 | 88 (97) | P. luminescens |
| lrhA1::Tn10 | 2809 | Regulator | LysR-type transcriptional regulator | 2e−151 | 86 (93) | P. luminescens |
| gshA1::Tn10 | 1264 | Metabolic | γ-Glutamate-cysteine ligase | 0.0 | 74 (87) | P. luminescens |
| pgi1::Tn10 | 3940 | Metabolic | Glucose-6-phosphate isomerase | 0.0 | 84 (92) | P. luminescens |
| purM1::Tn10 | 2673 | Metabolic | Phosphoribosylaminoimidazole synthetase | 2e−177 | 85 (91) | P. luminescens |
| yigL1::Tn10 | 0426 | Unknown | HAD-like hydrolase | 4e−126 | 80 (87) | P. luminescens |
| insB1::Tn10 | 3457 | Transposon | Transposase B | 3e−51 | 68 (80) | Vibrio vulnificus |
a
Open reading frame (ORF) designation from the X. nematophila genome (https://www.genoscope.cns.fr/agc/mage/).
b
Based on predicted function from BLAST search results.
c
Based on BLASTp search results against NCBI nonredundant protein sequences. Database accessed on 3 February 2009.
d
Data are presented as percent identity (percent similarity).
e
Organism on which percent homology is based. P. luminescens; Photorhabdus luminescens.