TABLE 5.

Selected phenotypes of X. nematophila lipase-deficient mutants^a

Strain genotype	Motilityb	Lipasec	Proteased	Hemolysind	Antibiotic productione
Wild-type	+	+	+	+	+
gshA1::Tn10	+	−	+	+	−
pgi1::Tn10	+	−	+	+	+
purM1::Tn10	+	−	+	++	+
yigL1::Tn10	+	−	+	+	+
insB1::Tn10	+	−	+	+	+

^a+, the activity was indistinguishable from that of the wild-type; −, the activity was not detected; ++, the activity was greater than that of the wild type. Experiments were conducted at least twice with at least two replicates per experiment.

^bSize of colony 24 h after inoculation on 0.25% agar plates.

^cQualitative evaluation of halo surrounding the bacterial colony, 3 days after inoculation on plates containing Tween 20, 40, or 60.

^dQualitative evaluation of halo surrounding the bacterial colony 3 days after inoculation on milk or sheep blood agar plates.

^eQualitative evaluation of halo of no growth surrounding the bacterial colony 24 h after inoculation with the tester bacterium (Bacillus subtilis).