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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1982 Dec;16(6):1157–1159. doi: 10.1128/jcm.16.6.1157-1159.1982

Methods for distinguishing gram-positive from gram-negative bacteria.

G M Carlone, M J Valadez, M J Pickett
PMCID: PMC272558  PMID: 6186691

Abstract

Lysis by KOH and hydrolysis of L-alanine-4-nitroanilide were compared with the Gram reaction of aerobic, microaerophilic, and anaerobic bacteria. Both tests correlated well with the Gram reaction with nonfermentative bacilli and Bacillus species, whereas they did not correlate with nonsporulating anaerobes. Only campylobacteria were KOH positive and L-alanine-4-nitroanilide and gram negative.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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