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DNase I footprinting assay. A radioactively labeled copARZ promoter fragment was mixed with the indicated concentrations of purified CopR in binding buffer before the reaction mixture was treated with DNase I. The digested and protected DNA fragments were separated on 8% denatured DNA sequencing gels along with copARZ promoter sequence ladders (G, A, T, and C). BSA indicates that 1,500 nM BSA was added to the binding mixture instead of CopR.
