Overexpression of vpsS and luxQ activates vps expression. Expression of vpsL in a vpsLp-lacZ reporter strain harboring either the vector pBAD or pVC0303, pVC1831, pVCA0709, pVCA0719, pchiS, pcqsS, pluxQ, pvarS, or pvpsS was examined. Overnight cultures were diluted 1:500 and grown for 24 h in LB medium supplemented with ampicillin and 0.2% arabinose to induce HK overexpression. The error bars indicate standard deviations of eight technical replicates. The results of one representative experiment of four biological replicates are shown.