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. 2009 Jun 12;191(16):5147–5158. doi: 10.1128/JB.00401-09

FIG. 4.

FIG. 4.

VpsS requires VpsR to activate vps expression. (A) Expression of vpsL in wild-type (Wt), ΔvpsR, vpsR(D59A), vpsR(D59E), ΔvpsT, and ΔvpsR ΔvpsT strains harboring a vpsLp-lacZ reporter carrying the vector (open bars) or pvpsS (filled bars). (B) Expression of vpsL, vpsR, and vpsT from a single-copy chromosomal vpsLp-lacZ, vpsRp-lacZ, or vpsTp-lacZ reporter in the wild-type strain harboring the vector (open bars) or pvpsS (filled bars). vpsS was overexpressed in LB medium supplemented with ampicillin and 0.2% arabinose. The error bars indicate standard deviations of eight technical replicates. The results of one representative experiment of four biological replicates are shown.