LuxO positively regulates vpsL, vpsR, and vpsT expression. Expression of vpsL, vpsR, and vpsT in wild-type (Wt) and ΔluxO strains harboring single-copy chromosomal vpsLp-lacZ, vpsRp-lacZ, and vpsTp-lacZ reporters and grown to exponential phase in LB medium was examined. The error bars indicate standard deviations of eight technical replicates. The results of one representative experiment of four biological replicates are shown.