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Severe chaining defect of cells lacking multiple LytM factors. Shown are representative micrographs of fixed cells of TB156 (ΔenvC ΔnlpD) (A), TD22 (Δlyt4 = ΔenvC ΔnlpD ΔygeR ΔyebA) (B), and TB171 (Δami3 = ΔamiA ΔamiB ΔamiC) (C). Cells were grown, processed, and imaged as described in the legend to Fig. 2. Bar, 4 μm.
