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. 2009 Jun 5;191(16):5216–5223. doi: 10.1128/JB.00390-09

FIG. 5.

FIG. 5.

Southern analysis of S. scabies 87-22 hopanoid mutants. Chromosomal DNAs from the wild-type and Δhop615-1 and Δhop615-7 mutant strains were digested with PstI and after transfer to nylon membrane were probed with a PCR-generated dioxigenin-11-dUTP-labeled fragment upstream of SCAB13001. Hybridization of the probe to wild-type and Δhop615-1 and Δhop615-7 DNAs resulted in visualization of the anticipated 1,888-bp (wild-type) and 2,716-bp (mutant) bands. Sizes of the DNA markers are indicated to the left in kb. Lanes: 1, wild-type S. scabies 87-22; 2, Δhop615-1 mutant; 3, Δhop615-7 mutant.