FIG. 5.

Localization of the P-ring protein FlgI in the B. burgdorferi flagellar motor, as determined by cryo-ET-derived models from transposon mutants. Panel A is a map of the Borrelia burgdorferi genetic locus encoding the P-ring protein FlgI and other flagellar proteins. Gold-colored genes are flagellum related, and hypothetical genes are shown in blue. The alternative sigma factor gene rpoS is shown in red. The insertion sites of three transposon mutants used in this study are shown. The dashed arrow indicates the predicted transcript of a multicistronic operon with a promoter upstream of flhO. Sections through 3-D asymmetric averages of flagellar motors from WT cells (B) or three mutants, T09P01H08 (C), T03TC042 (D), and T06TC173 (E), are shown. The corresponding surface renderings are from WT (F) or flgI mutant T09P01H08 (G and H) cells. The difference map for the WT and the flgI mutant reveals the P ring (colored beige), shown in the inset and in the context of the motor (H).