Properties of MotX and MotY tagged with mCherry. (A) Swimming phenotype of strains harboring motX-mCherry and motY-mCherry. The strains were spotted on a 0.25% semisolid plate. (B) Detection of MotX-mCherry and MotY-mCherry by immunoblotting using antibodies raised against mCherry. Lane 1, wild type (negative control); lane 2, mCherry (positive control); lane 3, S292 (motX::mCherry); lane 4, S293 (motY::mCherry); lane 5, S294 (ΔmotY motX::mCherry); lane 6, S295 (ΔmotX motY::mCherry). Arrows indicate the positions of signals corresponding to MotX-mCherry (54.1 kDa), MotY-mCherry (62.4 kDa), and mCherry (29.3 kDa).