TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Relevant genotype and/or phenotypea	Source or reference
Escherichia coli strains
DH5α-λpir	φ80dlacZΔM15 Δ(lacZYA-argF)U196 recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1/λpir	25
WM3064	thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir(wt)]	W. Metcalf, University of Illinois, Urbana-Champaign
Shewanella oneidensis strains
MR-1	Wild type	46
S208	MR-1 ΔfliF	This work
S182	MR-1 ΔmotX	This work
S257	MR-1 ΔmotY	This work
S256	MR-1 ΔmotX ΔmotY	This work
S258	MR-1 ΔmotX ΔpomAB	This work
S255	MR-1 ΔmotY ΔpomAB	This work
S1099	MR-1 ΔmotX ΔmotAB	This work
S1100	MR-1 ΔmotY ΔmotAB	This work
S305	MR-1 ΔmotX pBBR-motX, complementation of ΔmotX, Gmr	This work
S323	MR-1 ΔmotY pBBR-motY, complementation of ΔmotY, Gmr	This work
S292	MR-1 motX-mCherry, C-terminal fusion of motX to mCherry, Kmr	This work
S293	MR-1 motY-mCherry, C-terminal fusion of motY to mCherry, Kmr	This work
S296	MR-1 ΔfliF motX-mCherry, Kmr	This work
S297	MR-1 ΔfliF motY-mCherry, Kmr	This work
S295	MR-1 ΔmotX motY-mCherry, Kmr	This work
S294	MR-1 ΔmotY motX-mCherry, Kmr	This work
S166	MR-1 pomB-mCherry, C-terminal fusion of pomB to mCherry, Kmr	32
S265	MR-1 ΔmotX pomB-mCherry, Kmr	This work
S267	MR-1 ΔmotY pomB-mCherry, Kmr	This work
S266	MR-1 ΔmotXY pomB-mCherry, Kmr	This work
S1150	MR-1 ΔmotAB ΔmotXY pomB-mCherry, Kmr	This work
S165	MR-1 motB-mCherry, C-terminal fusion of motB to mCherry, Kmr	32
S268	MR-1 ΔpomAB ΔmotX motB-mCherry, Kmr	This work
S269	MR-1 ΔpomAB ΔmotY motB-mCherry, Kmr	This work
S291	MR-1 ΔmotX ΔmotY motB-mCherry, Kmr	This work
S1151	MR-1 ΔmotY motB-mCherry, Kmr	This work
S1152	MR-1 ΔmotX motB-mCherry, Kmr	This work
S1091	MR-1 ΔpomAB ΔmotAB motX-mCherry, Kmr	This work
S1093	MR-1 ΔpomAB ΔmotAB motY-mCherry, Kmr	This work
Plasmids
pBC SK+	pUC origin, PtaclacZα, Cmr	Stratagene
pBBR1-MCS5	ori pBBR oriT. broad-host-range vector, Plac, Gmr	19
pGP704Sac28Km	mobRP4+ori-R6K sacB, suicide plasmid for in frame deletions, Kmr	44
pJP5603	mobRP4+ori-R6K, suicide plasmid for mutation by plasmid integration, Kmr	33
pBBR-motX	motX in pBBR1-MCS5	This work
pBBR-motY	motY in pBBR1-MCS5	This work
pGP704Sac28Km-fliF	fliF in-frame deletion fragment in pGP704Sac28-Km, Kmr	This work
pGP704Sac28Km-motX	motX in-frame deletion fragment in pGP704Sac28-Km, Kmr	This work
pGP704Sac28Km-motY	motY in-frame deletion fragment in pGP704Sac28-Km, Kmr	This work
pJP5603-mCherry	Promoterless mCherry in pJP5603, Kmr	This work
pJP5603-motX::mCherry	C-terminal fusion of motX to mCherry in pJP5603, Kmr	This work
pJP5603-motY::mCherry	C-terminal fusion of motY to mCherry in pJP5603, Kmr	This work
pJP5603-motB::mCherry	C-terminal fusion of motB to mCherry in pJP5603, Kmr	32
pJP5603-pomB::mCherry	C-terminal fusion of pomB to mCherry in pJP5603, Kmr	32

^aCm^r, chloramphenicol resistance; Gm^r, gentamicin resistance; Km^r, kanamycin resistance.