TABLE 2.
Characteristics of the plasmids used in this study
| Plasmid | Relevant feature(s)a | Source or reference |
|---|---|---|
| pGEMT | AT overhang Ampr cloning vector | Promega |
| pUC4K | Source of the Kmr marker gene | Pharmacia |
| pHPΩ45 | Source of the Smr Spr marker gene | 32 |
| ppbp1 | pGEMT with the Synechocystis pbp1 gene and its flanking sequences, where part of the PBP1 CS (from bp 84 to 2424) was replaced by a SmaI site | This study |
| pΔpbp1::Kmr | ppbp1 with the Kmr marker inserted in the unique SmaI site | This study |
| pΔpbp1::SmrSpr | ppbp1 with the Smr Spr marker inserted in the unique SmaI site | This study |
| ppbp2 | pGEMT with the Synechocystis pbp2 gene and its flanking sequences, where part of the PBP2 CS (from bp 111 to 2037) was replaced by a SmaI site | This study |
| pΔpbp2::Kmr | ppbp2 with the Kmr marker inserted in the unique SmaI site | This study |
| pΔpbp2::SmrSpr | ppbp2 with the Smr Spr marker inserted in the unique SmaI site | This study |
| ppbp3 | pGEMT with the Synechocystis pbp3 gene and its flanking sequences, where part of the PBP3 CS (from bp 180 to 1734) was replaced by a SmaI site | This study |
| pΔpbp3::Kmr | ppbp3 with the Kmr marker inserted in the unique SmaI site | This study |
| pΔpbp3::SmrSpr | ppbp3 with the Smr Spr marker inserted in the unique SmaI site | This study |
| pftsI | pGEMT with the Synechocystis ftsI gene and its flanking sequences, where part of the FtsI CS (from bp 129 to 1701) was replaced by a SmaI site | This study |
| pΔftsI::Kmr | pftsI with the Kmr marker inserted in the unique SmaI site | This study |
| ppbp5 | pGEMT with the Synechocystis pbp5 gene and its flanking sequences, where part of the PBP5 CS (from bp 129 to 1326) was replaced by a SmaI site | This study |
| pΔpbp5::Kmr | ppbp5 with the Kmr marker inserted in the unique SmaI site | This study |
| ppbp6 | pGEMT with the Synechocystis pbp6 gene and its flanking sequences, where part of the PBP6 CS (from bp 78 to 1,098) was replaced by a SmaI site | This study |
| pΔpbp6::Kmr | ppbp6 with the Kmr marker inserted in the unique SmaI site | This study |
| ppbp7 | pGEMT with the Synechocystis pbp7 gene and its flanking sequences, where part of the PBP7 CS (from bp 129 to 1602) was replaced by a SmaI site | This study |
| pΔpbp7::Kmr | ppbp7 with the Kmr marker inserted in the unique SmaI site | This study |
| pΔpbp7::SmrSpr | ppbp7 with the Smr Spr marker inserted in the unique SmaI site | This study |
| ppbp8 | pGEMT with the Synechocystis pbp8 gene and its flanking sequences, where part of the PBP8 CS (from bp 72 to 876) was replaced by a SmaI site | This study |
| pΔpbp8::Kmr | ppbp8 with the Kmr marker inserted in the unique SmaI site | This study |
| pΔpbp8::SmrSpr | ppbp8 with the Smr Spr marker inserted in the unique SmaI site | This study |
| pftsQ | pGEMT with the Synechocystis ftsQ gene and its flanking sequences, where part of the FtsQ CS (from bp 138 to 663) was replaced by a SmaI site | This study |
| pΔftsQ::Kmr | pftsQ with the Kmr marker inserted in the unique SmaI site | This study |
| pftsW | pGEMT with the Synechocystis ftsW gene and its flanking sequences, where part of the FtsW CS (from bp 96 to 1029) was replaced by a SmaI site | This study |
| pΔftsW::Kmr | pftsW with the Kmr marker inserted in the unique SmaI site | This study |
| pKT25 | Kmr plasmid encoding the N-terminal T25 domain (amino acids 1-224) of the B. pertussis AC in frame with a downstream multiple cloning site | 16 |
| pKT25-zip | pKT25 with the leucine zipper domain of the yeast GCN4 activator | 16 |
| pKT25-zipN | pKT25 with the full-length Synechocystis zipN CS | This study |
| pKT25-ftsQ | pKT25 with the full-length Synechocystis ftsQ CS | This study |
| pKT25-ftsW | pKT25 with the full-length Synechocystis ftsW CS | This study |
| pKT25-ftsI | pKT25 with the full-length Synechocystis ftsI CS | This study |
| pKT25-pbp1 | pKT25 with the full-length Synechocystis pbp1 CS | This study |
| pKT25-pbp2 | pKT25 with the full-length Synechocystis pbp2 CS | This study |
| pKT25-pbp3 | pKT25 with the full-length Synechocystis pbp3 CS | This study |
| pKT25-pbp5 | pKT25 with the full-length Synechocystis pbp5 CS | This study |
| pKT25-pbp6 | pKT25 with the full-length Synechocystis pbp6 CS | This study |
| pKT25-pbp7 | pKT25 with the full-length Synechocystis pbp7 CS | This study |
| pKT25-pbp8 | pKT25 with the full-length Synechocystis pbp8 CS | This study |
| pUT18 | Ampr plasmid encoding the T18 domain (amino acids 225-339) of the B. pertussis AC in frame with an upstream multiple cloning site | 16 |
| pUT18-zip | pUT18 with the leucine zipper domain of the yeast GCN4 activator | 16 |
| pUT18m1 | pUT18 derivative encoding the AC T18 domain in frame with a downstream multiple cloning site identical to that of pKT25 | This study |
| pUT18m1-zipN | pUT18m1 with the full-length Synechocystis zipN CS | This study |
| pUT18m1-ftsQ | pUT18m1 with the full-length Synechocystis ftsQ CS | This study |
| pUT18m1-ftsW | pUT18m1 with the full-length Synechocystis ftsW CS | This study |
| pUT18m1-ftsI | pUT18m1 with the full-length Synechocystis ftsI CS | This study |
| pUT18m1-pbp1 | pUT18m1 with the full-length Synechocystis pbp1 CS | This study |
| pUT18m1-pbp2 | pUT18m1 with the full-length Synechocystis pbp2 CS | This study |
| pUT18m1-pbp3 | pUT18m1 with the full-length Synechocystis pbp3 CS | This study |
| pUT18m1-pbp5 | pUT18m1 with the full-length Synechocystis pbp5 CS | This study |
| pUT18m1-pbp6 | pUT18m1 with the full-length Synechocystis pbp6 CS | This study |
| pUT18m1-pbp7 | pUT18m1 with the full-length Synechocystis pbp7 CS | This study |
| pUT18m1-pbp8 | pUT18m1 with the full-length Synechocystis pbp8 CS | This study |
a
CS, protein coding sequence; Δ, deletion.