TABLE 5.
Strain
|
lacZ fusion | β-Galactosidase activity (MU)
|
Ratiob | ||
---|---|---|---|---|---|
tolC+ | tolC | tolC+ strain | tolC strain | ||
M3954 | M4188 | marRAB | 30 (2.8)c | 57 (12.7) | 1.9 |
M4014 | M4183 | soxS | 133 (12.3) | 358 (54) | 2.7 |
M4113 | M4117 | inaA::lacZd | 38 (6.7) | 85 (9.2) | 2.2 |
The strains were grown at 32°C to an A600 of 0.1 to 0.25 in M9 medium supplemented with 0.2% glucose, 1 ng/ml thiamine, and 0.2% Casamino Acids, and an assay was performed to determine the β-galactosidase activity, which was expressed in Miller units (MU).
Ratio of the activity in the tolC mutant to the activity in the wild-type strain.
The standard errors of the means are indicated in parentheses.
As in Table 2, the expression of inaA::lacZ was used to measure the posttranscriptional activation of Rob.