TABLE 5.

Effects of tolC::Tn10 on promoter transcription in minimal medium^a

Strain		lacZ fusion	β-Galactosidase activity (MU)		Ratiob
tolC+	tolC		tolC+ strain	tolC strain
M3954	M4188	marRAB	30 (2.8)c	57 (12.7)	1.9
M4014	M4183	soxS	133 (12.3)	358 (54)	2.7
M4113	M4117	inaA::lacZd	38 (6.7)	85 (9.2)	2.2

^aThe strains were grown at 32°C to an A₆₀₀ of 0.1 to 0.25 in M9 medium supplemented with 0.2% glucose, 1 ng/ml thiamine, and 0.2% Casamino Acids, and an assay was performed to determine the β-galactosidase activity, which was expressed in Miller units (MU).

^bRatio of the activity in the tolC mutant to the activity in the wild-type strain.

^cThe standard errors of the means are indicated in parentheses.

^dAs in Table 2, the expression of inaA::lacZ was used to measure the posttranscriptional activation of Rob.