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Organization of the kgt locus. The triangles indicate the location of theTn5 transposon in each mutant (GA11 and GA12). Restriction sites are abbreviated as follows: E, EcoRI; S, SalI; C, ClaI. The horizontal lines represent the chromosomal fragments cloned into the plasmids indicated above each line. The vectors used to clone the fragments of pSB10 and pSB11 were pCR 2.1 and pHRP317, respectively. For pSB12 and pSB13, the fragments were cloned into pMP220 in both cases.
