TABLE 1.
Bacterial strains and plasmids
| Strain, plasmid, phagemid, or cosmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| S17-1 | Smr Tpr derivative of E. coli 294 pro recA hsdR [RP4 (IncP):2 kan::Tn7 tet::Mu] in the chromosome | 31 |
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen Corp. |
| Rhizobium tropici | ||
| CIAT899 | Wild-type strain isolated from Phaseolus vulgaris L.; Nalr | 11 |
| GA1 | CIAT899 dctA::Gmr “cassette” | 5 |
| GA11 | GA1 kgtS::Tn5; Gmr Kmr | This work |
| GA12 | GA1 kgtR::Tn5; Gmr Kmr | This work |
| CIAT899.317 | CIAT899 kgtP::pHRP317; Kmr | This work |
| GA1.317 | GA1 kgtP::pHRP317; Gmr Kmr | This work |
| Plasmids, phagemids, and cosmids | ||
| pBluescript II SK | Phagemid and ColE1 replicon; Ampr | Stratagene |
| pSK-KG | Derived from pBluescript II SK with intergenic region kgtP-kgtS from R. tropici 1.2-kb, EcoRI-SalI site | This work |
| pCR 2.1-TOPO | pUC and f1 origin; Ampr Kmr | Invitrogen Corp. |
| pTH24 | 6-kb HindIII fragment with dctABD genes from S. meliloti cloned into pRK7813 | 39 |
| pRTD1 | Derived from pSUP205 with dct of R. tropici | 5 |
| pMP220 | IncP cosmid with lacZ as reporter gene; Tcr | 32 |
| pRU103 | Derived from pMP220 with intergenic region dctA-dctB from R. leguminosarum 0.9-kb EcoRI; dctA::lacZ; Tcr | 26 |
| pSUP202-1::Tn5 | Derived from pBR325 (ColE1) with oriT region; Kmr | 31 |
| pGA11-10 | Derived from pBluescript II SK with 10-kb fragment of EcoRI including Tn5 from GA11 | This work |
| pGA12-10 | Derived from pBluescript II SK with 10-kb fragment of EcoRI Including Tn5 from GA12 | This work |
| pHRP317 | pMB1 ori; Kmr Spcr Strr Kmr Smr; fQ Sm/Sp cassette from pHRP316 (MscI-EcoRI fragment) in HincII-EcoRI of pK19 | 21 |
| pSB10 | Derived from pCR 2.1 containing a PCR fragment from the internal region of the coding sequence of kgtP | This work |
| pSB11 | Derived from pHRP317 containing an internal fragment of the coding region of kgtP from pCR 2.1::KGT XhoI-SalI | This work |
| pSB12 | Derived from pMP220 with EcoRI-SalI fragment from pGA11-10 containing the presumptive ORF12 | This work |
| pSB13 | Derived from pMP220 with intergenic region kgtP-kgtS from R. tropici 1.5-kb KpnI-PstI fragment | This work |