TABLE 2.
Succinate transport activity in bacteria grown with different carbon sources
| Strain | Mean sp act of succinate uptake (nmol min−1 mg protein−1) ± SDa
|
|||
|---|---|---|---|---|
| Glucose | Glucose + 2-oxoglutarate | Succinate | 2-Oxoglutarate | |
| R. tropici CIAT899 | 0.7 ± 0.2 | ND | 26.3 ± 0.5 | 54.9 ± 0.7 |
| R. tropici GA1 (dctA) | 3.4 ± 0.2 | ND | 60.1 ± 0.6 | 51.7 ± 0.7 |
| R. tropici GA11 (dctA kgtS) | ND | 1.1 ± 0.2 | ND | ND |
| R. tropici GA12 (dctA kgtR) | ND | 1.2 ± 0.3 | ND | ND |
a
Succinate transport activity was measured in cells grown in MM with 20 mM of each carbon source for about 24 h, harvested, and suspended in MM without a carbon source at an optical density at 620 nm of 7.0. Each value represents the mean ± SD of data from two separate experiments. ND, not determined.