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. 2009 Jul 6;191(17):5489–5498. doi: 10.1128/JB.00590-09

FIG. 5.

FIG. 5.

Elevated amounts of Spo0A(A257V) cannot stimulate transcription of spoIIG-lacZ or spoIIA-lacZ promoter fusions in vivo. B. subtilis strains were grown in SSM, and samples were collected and harvested during the transition from an exponential to a stationary phase of growth; time zero indicates the end of exponential growth. (A) Whole-cell extracts were separated by electrophoresis through 12% SDS-PAGE, transferred onto nitrocellulose membranes, subjected to immunoblot analysis using an anti-Spo0A polyclonal antibody, and detected using a VersaDoc MP 4000 system. (B) Protein levels were quantified by densitometry using purified Spo0A as a standard. (C) Transcription of spoIIG-lacZ and spoIIA-lacZ promoter fusions in B. subtilis Spo0A overexpression strains was determined by assaying β-galactosidase activity in samples of cells grown in SSM. Closed circles, spo0A; closed triangles, spo0A crsA47; open circles, spo0A(A257V); open triangles, spo0A(A257V) crsA47.