TABLE 1.
Bacterial strains used in this study
| Strain | Description | Source/Reference |
|---|---|---|
| E. coli | ||
| DH5α | Cloning strain | Invitrogen |
| BL21(λDE3)(pLysS) | IPTGa-inducible protein over expression strain | Novagen |
| MC0A | Spo0A overexpression | 8 |
| BTA9V | Spo0A(A257V) overexpression | This study |
| B. subtilis | ||
| JH642 | trpC2 phe-1 | J.A. Hoch, Scripps Research Institute, La Jolla, Cal |
| JH695 | trpC2 phe-1 spo0A(A257V) | 11 |
| GBS101 | crsA47 trpC2 phe-1 amyE::(spoIIG-lacZ Kmr) | 9 |
| GBS106 | crsA47 trpC2 phe-1 amyE::(spoIIA-lacZ Cmr) | 9 |
| GBS1011 | JH695 crsA47 trpC2 phe-1 amyE::(spoIIG-lacZ Kmr) | This study |
| GBS1061 | JH695 crsA47 trpC2 phe-1 amyE::(spoIIA-lacZ Cmr) | This study |
| JH16304 | JH642 amyE::(spoIIG1-lacZ) Cmr | 31 |
| SS642IIG1 | JH642 amyE::(spoIIG1-lacZ) Cmr | This study |
| SS642IIG2 | JH642 amyE::(spoIIG1-lacZ) Cmr | This study |
| SS642IIG3 | JH642 amyE::(spoIIG1-lacZ) Cmr | This study |
| SS642IIG4 | JH642 amyE::(spoIIG1-lacZ) Cmr | This study |
| SS642IIG5 | JH642 amyE::(spoIIG1-lacZ) Cmr | This study |
| DR2001 | JH16304 amyE::(spoIIG-lacZ) spo0A(A257V) Cmr | 33 |
| SS695IIG1 | JH695 amyE::(spoIIG3-lacZ) Cmr | This study |
| SS695IIG2 | JH695 amyE::(spoIIG3-lacZ) Cmr | This study |
| SS695IIG3 | JH695 amyE::(spoIIG3-lacZ) Cmr | This study |
| SS695IIG4 | JH695 amyE::(spoIIG3-lacZ) Cmr | This study |
| SS695IIG5 | JH695 amyE::(spoIIG3-lacZ) Cmr | This study |
a
IPTG, isopropyl-β-d-thiogalactopyranoside.