TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source/reference |
|---|---|---|
| pGEM-T | PCR cloning vector | Promega |
| pGEMA9V | pGEM-T spo0A(A257V) gene | This study |
| pGEMSpo0A | pGEM-T with spo0A sequence from RBS to E263 | This study |
| pET16b | Protein expression vector | Novagen |
| pET16b0A | pET16b with an 848-bp NcoI-BamHI insert bearing the spo0A gene | 8 |
| pET16bA9V | pET16b with an 848-bp NcoI-BamHI insert bearing the spo0A(A257V) gene | This study |
| pUCIIGtrpA | pUC19 with spoIIG promoter and proximal transcript fused to trpA terminator | 36 |
| pJM5134 | Cloning vector with abrB promoter | 30 |
| pUCIIGAtrpASS1 | pUCIIGtrpA with a single mutation in site 2 0A boxes | This study |
| pUCIIGAtrpASS2 | pUCIIGtrpA with two mutations in site 2 0A boxes | This study |
| pUCIIGAtrpASS3 | pUCIIGtrpA with three mutations in site 2 0A boxes | This study |
| pUCIIGAtrpASS4 | pUCIIGtrpA with four mutations in site 2 0A boxes | This study |
| pUCIIGAtrpASS5 | pUCIIGtrpA with five mutations in site 2 0A boxes | This study |
| pDH32 | amyE integrative vector for lacZ fusions | 42 |
| pDH32SSIIG1 | pDH32 containing an EcoRI-BamHI fragment from pUCIIGtrpASS1 | This study |
| pDH32SSIIG2 | pDH32 containing an EcoRI-BamHI fragment from pUCIIGtrpASS2 | This study |
| pDH32SSIIG3 | pDH32 containing an EcoRI-BamHI fragment from pUCIIGtrpASS3 | This study |
| pDH32SSIIG4 | pDH32 containing an EcoRI-BamHI fragment from pUCIIGtrpASS4 | This study |
| pDH32SSIIG5 | pDH32 containing an EcoRI-BamHI fragment from pUCIIGtrpASS5 | This study |