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Summary of scheme to determine the spore proteome. (a) SDS-PAGE gel of vegetative cells (veg) (left lane) and pure spores (right lane) after solubilization with LDS. (b) Flow diagram outlining the steps required to solubilize proteins from C. difficile spores and the number of extra proteins liberated at each stage. (c) Rarefaction curve showing the decrease in the number of extra proteins with each additional solubilization step.
