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. 2009 Jun 19;191(17):5446–5457. doi: 10.1128/JB.00602-09

FIG. 1.

FIG. 1.

Location of the bsrF gene. (A) Schematic diagram of the location of the bsrF gene on the B. subtilis chromosome. The direction of transcription is indicated by arrows. Genes transcribed from the plus strand are indicated above the line. (B) Sequence of the bsrF gene. −35 and −10 boxes of the bsrF promoter are indicated. The start site and direction of transcription are indicated by an arrow, and the transcription termination site is indicated by term. The transcription start site is at position 2078305 in the B. subtilis genome. The small numbers indicate the positions of nucleotides referred to in Fig. 8. (C) Mapping of the 5′ end of BsrF. The results of a sequencing reaction with pUCBSR2 (containing the entire bsrF gene under its own promoter) and primer SB847 are shown in the left four lanes. The lane on the right shows the results of a primer extension reaction with total RNA of B. subtilis DB104 and 5′-labeled primer SB847. The arrow indicates the transcriptional start site of BsrF. Part of the sequence is shown on the left. The dot and the arrow indicate the mapped transcriptional start site. (D) Alignment of the bsrF genes of B. subtilis and B. amyloliquefaciens. Promoter boxes are indicated by boxes, and the transcription start site is underlined.