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In vitro transcription with B. subtilis RNA polymerase. In vitro transcription in the presence or absence of CodY was performed with B. subtilis RNA polymerase. BCAA and GTP were added at concentrations of 10 mM and 2 mM, respectively, where indicated. The template was prepared by performing PCR with oligonucleotides SB1233 and SB1064 and comprises the same bsrF upstream region as pACG6.
