FIG. 9.

Expression of the bsrF gene in B. amyloliquefaciens. B. amyloliquefaciens strain FZB42 was grown in TY or CSE medium, and aliquots were removed at hourly intervals at the OD₅₆₀ indicated, immediately frozen in liquid nitrogen, and later used for preparation of total RNA. Northern blotting was performed as described in the legend to Fig. 2 with the same BsrF probe. Autoradiograms of the corresponding gels are shown. ON, overnight cultivation (∼15 h). For correction of loading errors, filters were reprobed with [γ-³²P]ATP-labeled oligonucleotide SB1319 specific for B. amyloliquefaciens 23S rRNA.