TABLE 1.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pUC19 | E. coli cloning vector, Apr, multiple cloning site | 36 |
| pUCB2 | Shuttle vector for pUC19 and pUB110, Kmr Pmr | Brantl, unpublished data |
| pCAT | pUC19 with 1.4-kb CAT gene | 24 |
| pAC6 | Vector for integration of transcriptional lacZ fusions | 42 |
| pWH353 | E. coli-B. subtilis shuttle vector, Tet system, Kmr | 12 |
| pWSR2 | pWH353 with bsrF gene under tet-inducible promoter | This study |
| pUCBSR2 | pUCB2 with bsrF gene under its own promoter | This study |
| pFRONT5 | pUC19 with 800 bp upstream of bsrF | This study |
| pBACK5 | pUC19 with 500 bp downstream of bsrF | This study |
| pCBACK5 | pBACK5 with CAT gene from pCAT | This study |
| pINT5 | Vector for replacement of bsrF by CAT gene | This study |
| pACG6 | pAC6 with 415 bp upstream of bsrF transcription start site | This study |
| pACG9 | pAC6 with 375 bp upstream of bsrF transcription start site | This study |
| pACG10 | pAC6 with 302 bp upstream of bsrF transcription start site | This study |
| pACG12 | pAC6 with 71 bp upstream of bsrF transcription start site | This study |
| pACG13 | pAC6 with 89 bp upstream of bsrF transcription start site | This study |
| pACG16 | pAC6 with 58 bp upstream of bsrF transcription start site | This study |
| pACG17 | pAC6 with 48 bp upstream of bsrF transcription start site | This study |
| pACG4 | pACG6 with 35 bp upstream of bsrF transcription start site | This study |
| pACG11 | pACG6 with two point mutations in CodY binding site | This study |
| pACG15 | pACG6 with one point mutation in CodY binding site | This study |
| pACG18 | pACG6 with two point mutations in CodY binding site | This study |
| pACG19 | pACG6 with two point mutations in CodY binding site | This study |