TABLE 1.
Bacterial strains used
| Strain | Relevant genotypea | Source or referenceb |
|---|---|---|
| BW386 | recA56 srlD300::Tn10 | Laboratory collection |
| BW1177 | KD1092 trpA58 nfi-1::cat | 41 |
| BW1824 | CC106 tna+dgoA::Tn10dkan | This study |
| BW1996 | KD1092 uvrA277::Tn10 | P1(N3055) × KD1092c |
| BW1997 | KD1092 uvrA277::Tn10 ΔtnaA739::kan | P1(N3055) × BW2021c |
| BW2018 | RV ΔtnaA739::kan | P1(JW3686-7) × RV |
| BW2019 | RV tnaB::mini-Tn5-lac-tet/1 | P1(MT113) × RV |
| BW2020 | RV narG205::Tn10 | P1(RK5268) × RV |
| BW2021 | KD1092 ΔtnaA739::kan | P1(JW3686-7) × KD1092 |
| BW2022 | KD1092 ΔtnaA739::kan nfi-1::cat | P1(JW3686-7) × BW1175 |
| BW2023 | KD1092 recA56 srlD300::Tn10 | P1(BW386) × KD1092c |
| BW2024 | KD1092 ΔtnaA739::kan recA56 srlD300::Tn10 | P1(BW386) × BW2021c |
| CC106 | tnaA Δ(gpt-lac)5 (F′ lacZCC106)d | 14 |
| JCB387 | RV ΔnirB | 15 |
| JW3686-7 | ΔtnaA739::kan | 3 |
| KD1092 | trpA58 | 16 |
| MT113 | tnaB::mini-Tn5-lac-tet/1 | 4 |
| N3055 | uvrA277::Tn10 | CGSCe |
| RK5268 | narG205::Tn10 | 48 |
| RV | Δlac tna+ | 15 |
a
All strains are derivatives of E. coli K-12 and are F− λ− unless stated otherwise. cat, kan, and tet are genes specifying resistance to chloramphenicol, kanamycin, and tetracycline, respectively.
b
Transductions with phage P1 are described as follows: P1(donor) × recipient.
c
Tetracycline-resistant transductants were tested for UV sensitivity (recA).
d
The tnaA mutant genotype was found in this study (see Results).
e
CGSC, Coli Genetic Stock Center, Yale University (http://cgsc.biology.yale.edu).