TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′)a | Use |
|---|---|---|
| Rec-C F | GGGGGCATGCCCGAGATCAACGCCTATACG | Mutant construction |
| Rec-C R | GGGGCTCGAGGCAAGTGTGATTTCGTTGAACTG | Mutant construction |
| TetR F | GGGGCTCGAGATTGCTATCGCGTTGTGTTTTATC | Mutant construction |
| TetR R | GGGGGAGCTCCAATCCCATGTTCCAGCAATTTG | Mutant construction |
| Cmr F | GGGGCTCGAGCGTGTCCGAATAAATACCTGTG | Mutant construction |
| Cmr R | GGGGCTCGAGCGCGTGTCCGAATTTCTG | Mutant construction |
| S comp F | GGGGCTGCAGACTTGTTAATATTTGTACCGCATTGG | Complementation |
| S comp R | GGGGAAGCTTGATAATCACTGACGAACTGCCTAC | Complementation |
| opnS F | ATGGCAAGGCAGATGTTCAG | RT-PCR analysis |
| opnS R | ACAGACATCAGCCATGCTTC | RT-PCR analysis |
| recA F | GCGCTGAAATTCTATGCGTCTGTC | RT-PCR analysis |
| recA R | GCAGTTTCTGGATGCTCTTTCAGG | RT-PCR analysis |
a
Restriction sites are denoted by the underlined sequences.