. 2009 Jun 19;191(17):5510–5517. doi: 10.1128/JB.00562-09

TABLE 2.

Properties of various E. coli mutants that have a linear respiratory chain grown aerobically in glucose-limited chemostat conditions at a dilution rate of 0.15 h⁻¹^a

Mutant	q_O₂ {mmol (g [dry wt])⁻¹ h⁻¹}	q_glucose {mmol (g [dry wt])⁻¹ h⁻¹}	Y_glucose (g [dry wt]) (g Glc)⁻¹	q_acetate {mmol (g [dry wt])⁻¹ h⁻¹}	q_ATP {mmol (g [dry wt])⁻¹ h⁻¹}	H⁺/2e⁻
MB30 (cytochrome bd-I)	8.8 ± 1.3	3.2	0.26	1.5 ± 0.5	16.3 ± 2.2	2
MB37 (cytochrome bd-II)	11.1 ± 0.6	6.0	0.14	5.7 ± 0.7	16.6	0.2 ± 0.1
MB34 (cytochrome bo)	6.4 ± 0.4	2.3	0.36	0.4 ± 0.4	17.0 ± 0.4	4

q_ATP values were calculated as described in Materials and Methods with an H⁺/2e⁻ stoichiometry of 2 for strain MB30 and of 4 for strain MB34, respectively. The average q_ATP value was then used to calculate the H⁺/2e⁻ stoichiometries for strain MB37. Other fluxes were determined as described in Materials and Methods. Strain MB37 produced lactate with a q_lactate of 0.8 mmol g (dry weight)⁻¹ h⁻¹. The carbon balances were 96% ± 4% (n = 3). q_glucose, rate of synthesis of glucose; Y_glucose, yield on glucose.