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Inactivation of slyA attenuates the virulence of D. dadantii 3937. The bacterial inoculum of 20 μl contained 104 CFU/ml of the wild type (D. dadantii 3937), the ΔslyA mutant, and the complementation strain [ΔslyA(pSlyA)]. The disease symptoms (A), mass of the rotted tissues (B), and bacterial populations in macerated tissue (C) were documented after 36 h of incubation at 27°C with high relative humidity. The error bars indicate standard deviations.
