TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| D. dadantii | ||
| 3937 | Wild type; Nalr | Laboratory collection |
| ΔslyA | ΔslyA mutant; Nalr | This study |
| 3937ΔslyA | pDEL-SlyA into D. dadantii 3937; Nalr Apr Stmr | This study |
| ΔslyA(pSlyA) | pSlyA into ΔslyA mutant; Nalr Apr | This study |
| MH70 | Km-inserted phoP mutant; Nalr Kmr | 18 |
| MH72 | Km-inserted phoQ mutant; Nalr Kmr | 18 |
| E. coli | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 (note the roman M15 after lacZΔ) | TaKaRa, Japan |
| CC118 | Δ(ara, leu) araD ΔlacX74 galE galK phoA20 thi-1 rps rpoB argE(Am) recA1 Spr | 19 |
| CC118 (λpir) | CC118 lysogenized with λpir phage; Spr | 19 |
| S17-1 | recA thi pro hsdR-M+RP4:2-Tc:Mu:km Tn7 λpir Smr | Biomedal |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Nippon gene |
| Plasmids | ||
| pGEM-T Easy | A-T cloning vector; Apr | Promega |
| pMRS101 | R6K origin sacB marker exchange vector; mob+, Apr Stmr | 51 |
| pSlyX | 2.34-kb ApaI-SacI fragment cloned into same site of pGEM-T Easy; Nalr Apr | This study |
| pSlyY | 0.911-kb ApaI-SmaI fragment upstream from slyA ORF cloned into same site of pGEM-T Easy, Nalr, Apr | This study |
| pSlyZ | 0.999-kb SmaI-SacI fragment downstream from slyA ORF cloned into same site of pGEM-T Easy; Nalr Apr | This study |
| pSlyYZ | 1.91-kb ApaI-SacI fragment contained deletion of slyA ORF cloned into same site of pGEM-T Easy; Nalr Apr | This study |
| pDEL-SlyA | 1.75-kb ApaI-SpeI fragment containing deletion of slyA ORF cloned into same site of pMRS101, Nalr, Apr, Stmr | This study |
| pSlyA | 1.29-kb fragment containing entire slyA gene with its promoter cloned into pGEM-T Easy; Nalr Apr | This study |
| pET28a(+) | T7 expression vector; Kmr | Novagen |
| pETSlyA | 0.44-kb fragment containing entire slyA gene in pET28a(+) | This study |
| pGEM-T | A-T cloning vector; Apr | Promega |
| pGEMphoP | 0.29-kb phoP promoter fragment in pGEM-T | Promega |