TABLE 2.

Molecular weights of GST-CtrA protein-DNA complexes, as determined by EMSA

Bound DNA	Mol wt of protein plus mol wt of DNA (103)	Multiple (protein monomers)a
Cori binding sites a and b, lower band	132 ± 12	2.0 ± 0.3
Cori binding sites a and b, upper band	234 ± 8	3.9 ± 0.2
Cori binding site c	135 ± 14	2.1 ± 0.3
Cori binding site e	153 ± 17	2.3 ± 0.4
Wild-type P1	140 ± 14	2.2 ± 0.2
P1 m3	138 ± 15	2.2 ± 0.2
Wild-type P2, lower band	144 ± 15	2.3 ± 0.3
Wild-type P2, upper band	227 ± 20	3.9 ± 0.4
P2 m3	135 ± 15	2.1 ± 0.2
P2 N7	147 ± 15	2.3 ± 0.3

^aMultiples were determined as follows: [(molecular weight of protein + molecular weight of DNA) − molecular weight of DNA]/(52 × 10³).