TABLE 2.
Oligonucleotides
| Primer | Sequence (5′-3′)a | Restriction site(s) |
|---|---|---|
| 4 | ATCAACAACGCACCACT | |
| 5 | ACCCAAGTCAAGCTGAT | |
| 241 | AGGAAACATTGGCACAATTTCAAAC | |
| 242 | CATGGATCCCTTTAGACATTTTATAACTCC | BamHI |
| 243 | CATGGATCCCAATCGTTAATCCTAAAACC | BamHI |
| 244 | ACGAATAAGTTCACAGTTGCTGCAA | |
| 266 | GGAGAATTCAAGCTTTGTAGATCCTGTTGCGGGAG | EcoRI, HindIII |
| 267 | GGACTGCAGTTGCAGAGTTACCTTCTGAAC | PstI |
| 289 | GGTGGTTCAGGCTTTGACAACGATACTAAATTTGA | |
| 247 | TAGTGAACCATTCCAGACTCAGTTTGG | |
| 291 | AAAGCCTGAACCACCTTGTGGAGCACCAGTAGGCG | |
| 246 | CAGGCTCAAGAAGACTTGCGAAACTC | |
| 342 | CTACTGCAAGCGGGAAGGATCCTTTGGCGT | BamHI |
| 344 | ACGCCAAAGGATCCTTCCCGCTTGCAGTAG | BamHI |
| 343 | CGACGGCCGTTAACGATTGTTAGAATCGTC | |
| 301 | TCAAAAGATTCAATGTGGCCTTG |
a
Restriction sites are indicated in boldface.