TABLE 1.
Bunyaviruses that are targeted and amplified by the molecular consensus assaya
| Genus/serogroup | Virusb | Strain | Source, location, and yr of isolation | RT-PCR result/serological identity confirmed by nucleotide sequencing and BLAST analysis | Detection limit (PFU/0.1 ml)c |
|---|---|---|---|---|---|
| Orthobunyavirus | |||||
| Bunyamwera | Bunyamwera virus | Original | Aedes spp., Uganda, 1943 | POS/yes | 70 |
| Cache Valley virus | 6V633 | Culiseta inornata, Utah, 1956 | POS/yes | 600 | |
| Fort Sherman virus* | 86MSP18 | Human, Panama, 1985 | POS/yes | 100 | |
| Ilesha virus | KO/2 | Human, Nigeria, 1957 | POS/no | 300 | |
| Shokwe virus* | Sa Ar 4042 | Aedes cumminsii, South Africa, 1962 | POS/yes | 75 | |
| Wyeomyia virus* | Original | Wyeomyia melanocephala, Colombia, 1940 | POS/yes | 660 | |
| Xingu virus* | BeH 388464 | Human, Brazil, unknown date | POS/yes | 4 | |
| Ngari (Garissa) virus | Not evaluated | Not evaluated | ND | ND | |
| Germiston virus | Not evaluated | Not evaluated | ND | ND | |
| Maguari virus | 75V3429 | Aedes scapularis, Ecuador, 1974 | POS/no | ND | |
| Potosi virus | 89-3380 | Aedes albopictus, Missouri, 1989 | POS/yes | ND | |
| Northway virus | 234 | Aedes spp., Alaska, 1971 | POS/yes | ND | |
| Biroa virus | DakArB 2198 | Anopheles pharoensis, CAR, 1969 | POS/yes | ND | |
| Batai virus | 184 | Anopheles maculipennis, Czechoslovakia, 1950 | POS/yes | ND | |
| Bwamba | Bwamba virus* | M 459 | Human, Uganda, 1937 | POS/yes | 200 |
| Pongola virus* | Sa Ar 1 | Aedes circumluteolus, South Africa, 1955 | POS/yes | 200 | |
| Simbu | Oropouche virus | TRVL 9760 | Human, Tobago, 1955 | POS/yes | 0.1 |
| California | California encephalitis virus | BFS 283 | Aedes melanimon, California, 1944 | POS/yes | 6 |
| Inkoo virus | KN 3641 | Aedes communis punctor, Finland, unknown date | POS/yes | 1.3 | |
| Jamestown Canyon virus | 61V2235 | Culiseta inornata, Colorado, 1967 | POS/yes | 60 | |
| La Crosse virus | Original | Human, Wisconsin, 1964 | POS/yes | 200 | |
| Snowshoe hare virus | LEIV 13004AKH | Aedes communis; USSR, 1986 | POS/no | 34 | |
| Tahyna virus | Bardos 92 | Aedes caspius, Czechoslovakia, 1958 | POS/yes | 340 | |
| Serra do Navio virus | BeAr 103645 | Aedes spp., Brazil, 1966 | POS/yes | ND | |
| South River virus | NJ0-94F | Anopheles crucians, New Jersey, 1966 | POS/yes | ND | |
| Jerry Slough virus | BFS 4474 | Culiseta inorata, California, 1963 | POS/yes | ND | |
| Nairovirus | |||||
| CCHF virus | CCHF virus | S segment cDNA | Plasmid-derived PCR control | POS/yes | ND |
| Hazara virus | PakJC 280 | Ixodes redikorzevi, Pakistan, 1964 | POS/yes | ND | |
| NSD | Dugbe virus | IbAr 1792 | Amblyomma variegatum, Nigeria, 1964 | POS/yes | 2 |
| Nairobi sheep disease virus | RV082 | RNA control from Kenyan strain | POS/yes | ND | |
| Phlebovirus (SF) | Alenquer virus* | BeH 301101 | Human, Brazil, 1976 | POS/yes | 0.02 |
| Chandiru virus* | BeH 22511 | Human, Brazil, 1960 | POS/yes | 0.3 | |
| Chagres virus* | JW 10 | Human, Panama, 1960 | POS/yes | 0.0024 | |
| Punta Toro virus | D 4021A | Human, Panama, 1966 | POS/yes | 32 | |
| Rift Valley fever virus | MP 12 | Vaccine strain | POS/yes | 100 | |
| Sandfly fever Naples virus | Original | Human, Naples, Italy, 1944 | POS/yes | 0.23 | |
| Toscana virus | IssPhl 3 | Phlebotomus perniciosus, Tuscany, Italy, 1971 | POS/yes | 0.95 | |
| Sandfly fever Sicilian virus | Original | Human, Sicily, Italy, 1943 | POS/yes | 0.0045 | |
| Cacao virus* | VP 437R | Lutzomyia trapidoi, Panama, 1970 | POS/yes | ND | |
| Buenaventura virus | CoAr 3319 | Lutzomyia spp., Colombia, 1964 | POS/yes | ND | |
| Frijoles virus | VP 161A | Lutzomyia spp., Panama, 1969 | POS/yes | ND | |
| Itaituba virus | bean 213542 | Didelphis masupialis, Brazil, 1971 | POS/yes | ND | |
| Itaporanga virus* | Original | Sentinal mouse, Brazil, 1962 | POS/yes | ND | |
| Gabek Forest virus* | SudAn 754-61 | Acomys albigena, Sudan, 1961 | POS/yes | ND | |
| Turuna virus* | Bear352492 | Lutzomyia spp., Brazil, 1978 | POS/yes | ND | |
| Rio Grande virus* | TBM3-204 | Neotoma micropus, Texas, 1973 | POS/yes | ND | |
| Nique virus* | 9c | Lutzomyia panamensis, Panama, 1972 | POS/yes | ND |
a
Known human pathogens appear in bold. NSD, Nairobi sheep disease virus group; SF, Sandfly fever virus group; CAR, Central African Republic; POS, positive; PFU, PFU equivalent; ND, not determined.
b
Ngari and Germiston viruses were not evaluated due to a lack of availability (Ngari virus) and handling restrictions (Germiston virus). *, S segment sequence data were determined for this virus prior to this study.
c
PFU equivalent determined through plaque titration of virus dilutions in Vero cells.