TABLE 3.
Oligonucleotide primers for the amplification and sequencing of the partial S segments of viruses of the family Bunyaviridae
| Targeted genus/genomic target | Forward primer
|
Reverse primer
|
Approx. amplicon size (bp) | ||
|---|---|---|---|---|---|
| Name | Sequence | Name | Sequence | ||
| Orthobunyavirus N ORF | Cal/Bwa group forward | GCAAATGGATTTGATCCTGATGCAG | Cal/Bwa group reverse | TTGTTCCTGTTTGCTGGAAAATGAT | 210 |
| Bun group forward | CTGCTAACACCAGCAGTACTTTTGAC | Bun group reverse | TGGAGGGTAAGACCATCGTCAGGAACTG | 250 | |
| Wyeomyia forward | ATGTCTGAAATTGTATTTGATGATATTGG | Wyeomyia reverse | TATTTCGATTCCCCGGAAAGT | 230 | |
| Oropouche forward | GGCCCATGGTTGACCTTACTTT | Oropouche reverse | ACCAAAGGGAAGAAAGTGAAT | 300 | |
| Nairovirus/phlebovirus | |||||
| Nairovirus S segment N ORF | Nairo forward | TCTCAAAGAAACACGTGCCGC | Nairo reverse | GTCCTTCCTCCACTTGWGRGCAGCCTGCTGGTA | 400 |
| Phlebovirus N ORF | Phlebo forward 1 | TTTGCTTATCAAGGATTTGATGC | Phlebo reverse | TCAATCAGTCCAGCAAAGCTGGGATGCATCAT | 370 |
| Phlebo forward 2 | TTTGCTTATCAAGGATTTGACC | ||||