TABLE 1.
Analytical sensitivities of the dPCR assay for detection of B.quintana, B.henselae, and C.burnetii
| Test format | Organism | No. of copies/reaction in pooled pathogen-free FFPE tissue samples (n = 3) with the following no. of copies of plasmids/reaction:
|
Analytical sensitivity
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 10 | 100 | 1,000 | 10,000 | No. of copies/reaction | No. of copies/g tissue | ||
| Monoplex | B.quintana | 0 | 2 | 3 | 3 | 3 | 3 | 10 | 80 |
| B.henselae | 0 | 2 | 3 | 3 | 3 | 3 | 10 | 80 | |
| C.burnetii | 0 | 3 | 3 | 3 | 3 | 3 | 1 | 8 | |
| Duplex | B.quintana | 0 | 0 | 3 | 3 | 3 | 3 | 10 | 80 |
| B.henselae | 0 | 1 | 3 | 3 | 3 | 3 | 10 | 80 | |
| C.burnetii | 0 | 0 | 3 | 3 | 3 | 3 | 10 | 80 | |