TABLE 2.
Sequences of the primers and probes used in genotype-specific RT-PCRs designed to detect HPV42, HPV43, and HPV44/55
| HPV genotype | Primer/probea | Sequence (5′-3′)b | Nucleotide positionc |
|---|---|---|---|
| HPV42 | 42F | GGTGACTGCCCACCATTAGA(s) | 6374-6393 |
| 42R | CCTCAGCAGACATTTTTAAGTAATCA(a) | 6548-6523 | |
| 42FL | AGTTTTATTCAGGATGGGGATATGGTGG-FL(s) | 6404-6431 | |
| 42LC | LC610-TGTAGGGTTTGGGGCACTAGATTTTGG(s) | 6433-6459 | |
| HPV43 | 43F | AACTTACCCAGTTTCCCTTAGG(s) | 7126-7147 |
| 43R | ACAACCCATACAGGTACAAAACA(a) | 7303-7281 | |
| 43FL | AACTGTAAAGCGTTCTGCACCATCC-FL(s) | 7196-7220 | |
| 43LC | LC640-CCTCTACGTCTGCCCCTGCCT(s) | 7222-7242 | |
| HPV44/55 | 44/55F | GGCCTAGTGAAAACCAGGTATATG(s) | 5701-5724 |
| 44/55R | AGTGTCTTGTTTGCTGGTCGT(a) | 5866-5846 | |
| 44/55FL | CTCCCGCCCCAGTATCCAAAG-FL(s) | 5731-5751 | |
| 44/55LC | LC705-AATAMCTACGGATGCCTATGTCAAACGCAC(s) | 5753-5782 |
a
HPV genotype-specific forward (F) and reverse (R) primers and hybridization probes (FL and LC; see footnote b) are indicated.
b
FL, fluorescein; LC610, LC640, and LC705, LightCycler red dyes; M, A or C; (s), sense orientation; (a), antisense orientation.