TABLE 1.
Summary of assay performance results with specimens from acutely infected and recently infected individualsa
| Sample ID | Initial screen | First-gen or second-gen EIA
|
WB | Third-gen EIA
|
OQ | UG | SR | MS | Viral load (copies/ml)b | Fourth-gen IA
|
|||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Avg S/C | Result | Avg S/C | Result | RT | RT | RT | RT | Avg S/CO | Result | ||||
| A | First-gen EIA | 0.351 | NR | I | 0.127 | NR | N | N | N | N | 5,770† | 0.37 | NR |
| B | First-gen EIA | 0.602 | NR | I | 0.955 | NR | N | N | N | N | ≥500,000† | 611.12 | R |
| C | First-gen EIA | 0.440 | NR | I | ≥14.658 | R | N | R | N | N | 12,183† | 1.62 | R |
| E | First-gen EIA | 0.368 | NR | I | 0.233 | NR | N | N | N | N | 6,373† | 0.65 | NR |
| F | First-gen EIA | 0.329 | NR | I | 13.433 | R | N | R | N | R1 | ≥500,000† | 85.73 | R |
| G | First-gen EIA | 0.317 | NR | N | 0.084 | NR | N | N | N | N | 12,852† | 0.74 | NR |
| H | First-gen EIA | 0.338 | NR | I | 0.109 | NR | N | N | N | N | 14,062† | 0.68 | NR |
| I | First-gen EIA | 0.646 | NR | I | ≥14.658 | R | R | R | R | R1 | ≥500,000† | 67.70 | R |
| J | First-gen EIA | 0.358 | NR | N | 0.106 | NR | N | N | N | N | 3,921† | 0.23 | NR |
| K | First-gen EIA | 0.346 | NR | N | 4.574 | R | N | R | N | N | ≥500,000† | 43.92 | R |
| L | First-gen EIA | 0.373 | NR | N | 0.175 | NR | N | R | N | N | ≥500,000† | 39.55 | R |
| M | OQ RT-FS | 0.344 | NR | N | 1.5327 | R | N | N | N | N | ≥500,000† | 368.21 | R |
| N | First-gen EIA | 0.337 | NR | N | 0.113 | NR | N | N | N | N | 1,177† | 0.21 | NR |
| O | OQ RT-FS | 0.301 | NR | N | 0.127 | NR | N | N | N | N | ≥500,000† | 61.32 | R |
| P | First-gen EIA | 0.755 | NR | N | ≥14.658 | R | N | R | N | R1 | ≥500,000† | 136.62 | R |
| Q | First-gen EIA | 0.311 | NR | N | 0.277 | NR | N | N | N | N | 43,173† | 1.80 | R |
| R | OQ RT-FS | 0.642 | NR | I | 0.117 | NR | N | N | N | N | 30,734† | 2.05 | R |
| S | First-gen EIA | 0.406 | NR | N | 13.276 | R | N | R | N | R1 | ≥500,000† | 219.97 | R |
| T | OQ RT-OF | 0.401 | NR | N | 4.929 | R | N | N | N | N | ≥500,000† | 268.30 | R |
| U | OQ RT-FS | 0.325 | NR | N | 0.195 | NR | N | N | N | N | ≥500,000† | 317.71 | R |
| V | OQ RT-FS | 0.512 | NR | N | 0.198 | NR | N | N | N | N | ≥500,000† | 20.53 | R |
| W | First-gen EIA | 0.504 | NR | I | ≥12.403 | R | N | R | N | R1 | ≥500,000† | 121.68 | R |
| Y | OQ RT-FS | 0.340 | NR | N | ≥12.590 | R | N | N | N | N | ≥500,000† | 237.06 | R |
| Z | First-gen EIA | 0.378 | NR | N | 0.201 | NR | N | N | N | N | 102,288† | 2.09 | R |
| AA | First-gen EIA | 0.343 | NR | N | 0.327 | NR | N | N | N | N | 327,333† | 6.34 | R |
| AB | First-gen EIA | 0.373 | NR | N | 0.189 | NR | N | N | N | N | ≥500,000† | 168.87 | R |
| AC | OQ RT-FS | 0.396 | NR | I | 0.280 | NR | N | N | N | N | ≥500,000† | 35.93 | R |
| AD | First-gen EIA | 0.426 | NR | N | 0.371 | NR | N | N | N | N | ≥500,000† | 132.59 | R |
| AE | OQ RT-FS | 0.369 | NR | N | 0.145 | NR | N | N | N | N | 389,850† | 12.24 | R |
| AF | OQ RT-FS | 0.761 | NR | I | 0.907 | NR | N | R | N | R1 | 413,186† | 13.89 | R |
| AG | Third-gen EIA | 0.436 | NR | I | 0.165 | NR | N | N | N | N | 446,770† | 19.21 | R |
| AH | Third-gen EIA | 0.371 | NR | N | 0.195 | NR | N | N | N | N | 358,030† | 10.66 | R |
| AJ | OQ RT-FS | 0.6 | NR | N | 1.528 | R | N | N | N | N | ≥500,000† | 24.25 | R |
| AK | OQ RT-FS | 0.6 | NR | N | 0.147 | NR | N | N | N | N | 427,490† | 3.45 | R |
| AM | OQ RT-FS | 0.41 | NR | N | 0.187 | NR | N | N | N | N | ≥500,000† | 309.58 | R |
| AN | OQ RT-OF | 0.33 | NR | N | 0.17 | NR | N | N | N | N | ≥500,000† | 17.55 | R |
| AO | Third-gen EIA | 0.45 | NR | N | 9.634 | R | N | N | N | N | ≥500,000† | 22.83 | R |
| AP | Third-gen EIA | 0.34 | NR | N | ≥12.834 | R | N | R | N | R1 | ≥500,000† | 131.50 | R |
| AR | OQ RT-OF | 0.45 | NR | N | 0.097 | NR | N | N | N | N | ≥500,000† | 10.57 | R |
| AS | Third-gen EIA | 0.48 | NR | I | ≥14.641 | R | R | R | R | R1 | ≥500,000† | 24.74 | R |
| AT | OQ RT-OF | 0.40 | NR | N | 0.174 | NR | N | N | N | N | ≥500,000† | 176.23 | R |
| AU | OQ RT-OF | 1.48 | R | P | 13.09 | R | R | R | R | R1 | 109,211 | 31.35 | R |
| AV | OQ RT-OF | 0.5 | NR | N | 0.271 | NR | N | N | N | N | 333,066 | 5.68 | R |
| AW | OQ RT-OF | 5.82 | R | P | 12.496 | R | R | R | R | R1 | 335 | 14.33 | R |
| AX | OQ RT-OF | 0.36 | NR | N | 1.554 | R | N | R | N | N | ≥10,000,000 | 360.32 | R |
| AY | OQ RT-OF | 0.45 | NR | N | 0.215 | NR | N | N | N | N | 69,599 | 1.46 | R |
| AZ | OQ RT-OF | 0.59 | NR | P | 9.454 | R | R | R | R | R1 | 2,915,309 | 80.82 | R |
| BA | OQ RT-OF | 0.31 | NR | N | 13.205 | R | N | R | N | R1 | 518,434 | 9.13 | R |
| BB | Third-gen EIA | 0.32 | NR | N | 0.16 | NR | N | N | N | N | 317,609 | 3.81 | R |
| BC | OQ RT-OF | 6.65 | R | P | ≥13.943 | R | R | R | R | R1 | 13,204 | 77.09 | R |
| BD | OQ RT-FS | 0.5 | NR | P | 10.7 | R | R | R | R | R1 | 8,887,199 | 128.99 | R |
| BE | OQ RT-FS | 0.38 | NR | I | 0.243 | NR | N | N | N | N | ≥10,000,000 | 132.66 | R |
| BF | OQ RT-OF | 0.34 | NR | N | 5.443 | R | N | N | N | N | ≥10,000,000 | 430.39 | R |
| BG | OQ RT-OF | 5.69 | R | P | ≥13.514 | R | R | R | R | R1 | 468,809 | 12.66 | R |
| BH | OQ RT-OF | 0.35 | NR | N | 0.232 | NR | N | N | N | N | 9,289,006 | 80.34 | R |
| BI | OQ RT-FS | 2.93 | R | P | 10.564 | R | R | R | R | R1 | 355 | 3.99 | R |
| BJ | Third-gen EIA | 0.346 | NR | I | 0.123 | NR | N | N | N | N | 9,855 | 0.71 | NR |
| BK | OQ RT-OF | 0.294 | NR | N | 0.403 | NR | N | N | N | N | 650,629 | 6.08 | R |
| BL | OQ RT-OF | 5.13 | R | P | ≥14.493 | R | R | R | R | R1 | 752 | 199.51 | R |
| BM | OQ RT-FS | 0.326 | NR | N | 0.345 | NR | N | N | N | N | 4,589,912 | 44.06 | R |
| BN | OQ RT-FS | 2.959 | R | I | 1.18 | R | R | N | R | R1 | 4,571,787 | 48.35 | R |
| BO | OQ RT-FS | 0.22 | NR | N | 0.168 | NR | N | N | N | N | 1,531,891 | 18.05 | R |
| BP | OQ RT-FS | 1.015 | R | N | 4.139 | R | N | R | N | R1 | ≥10,000,000 | 136.82 | R |
| BQ | OQ RT-FS | 0.15 | NR | N | 0.539 | NR | N | N | N | N | 3,427,483 | 14.92 | R |
a
The first-generation (first-gen) EIA was the BioMérieux Vironostika HIV-1 micro-ELISA. The second-generation EIA was the Bio-Rad Genetic Systems rLAV HIV-1 EIA. The third-generation EIA was the Bio-Rad Genetic Systems HIV-1/2 plus O EIA. The fourth-generation IA was the Abbott Architect HIV Ag/Ab Combo. WB, Western blot assay using Bio-Rad Genetic Systems HIV-1; OQ RT-OF, OraQuick rapid test of oral fluid; OQ RT-FS, OraQuick rapid test of fingerstick sample; OQ-RT, Oraquick Advance HIV-1/2 rapid test; UG -RT, Uni-Gold Recombigen HIV-1 rapid test; SP-RT, Stat Pak HIV-1/2 rapid test; MS-RT, Multi-Spot HIV-1/2 rapid test. Results: R, reactive; NR, nonreactive; R1, reactive for HIV-1; P, positive; N, negative; I, indeterminate.
b
†, viral load was determined with Siemens Versant HIV-1 RNA v. 3.0 (otherwise, samples were tested using the Abbott real-time HIV-1 test).