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. 2009 Jun 22;29(17):4729–4741. doi: 10.1128/MCB.00289-09

FIG. 2.

FIG. 2.

Characterization of C-terminal and N-terminal deletion mutants of FLASH. (A and B) 293T cells were transfected with an empty vector (−) or an expression vector encoding mFLASH-FLAG3, ΔCC mFLASH-FLAG3, or ΔN194 mFLASH-FLAG3 together with that encoding mFLASH-EGFP. After 48 h of cultivation, mFLASH immunoprecipitates (IP) obtained with anti-Flag agarose resin or anti-GFP Ab were analyzed by Western blotting (WB) with anti-Flag and anti-GFP Abs. (C) 293T cells were transfected with empty vector (−) or an expression vector encoding mFLASH-EGFP, ΔN mFLASH-EGFP, ΔC1 mFLASH-EGFP, or ΔC2 mFLASH-EGFP. After 48 h of cultivation, immunoprecipitates obtained with anti-GFP Ab were analyzed by Western blotting with anti-GFP or anti-NPAT Ab. Representative results from three independent experiments are shown. Arrows indicate various deletion mutants of FLASH, and asterisks indicate nonspecific bands.