Skip to main content
. 2009 Jun 22;29(17):4729–4741. doi: 10.1128/MCB.00289-09

FIG. 3.

FIG. 3.

Interaction of FLASH with NPAT is not required for S phase progression. (A and B) KB cells with Tet-On shFLASH expressing or not expressing mFLASH-EGFP were cultured with (+) or without (−) 1 μg/ml Dox for 3 days, and NPAT (A) or coilin (B) was stained with anti-NPAT and anticoilin Ab, respectively. NPAT or coilin (red) and EGFP (green) were observed under a confocal fluorescence microscope. DNA was stained with DAPI (blue). Scale bars, 5 μm. (C and D) The graphs on the left show the percentages of cells (n > 100) with NPAT foci (C) and coilin foci (D), which were determined in KB cells as described for panels A and B, respectively. The graphs on the right show the percentages of cells with NPAT foci or coilin foci together with FLASH foci (EGFP foci), which were determined in KB cells as described in the legend to Fig. 1E. Representative results from three independent experiments, all performed in triplicate, are shown. Error bars denote ± standard errors of the means.