TABLE 1.
Characterization of various mutants of FLASHa
| mFLASH type | Presence of Dox | Nuclear focus formation
|
Expression of histone mRNA | Cell cycle progression | ||
|---|---|---|---|---|---|---|
| FLASH | NPAT | Coilin | ||||
| Exogenous mFLASH | − | ++ | ++ | ++ | ++ | |
| Exogenous mFLASH | + | − | − | ± | − | |
| mFLASH | + | ++ | ++ | ++ | ++ | ++ |
| ΔCC mFLASH | + | − | − | − | ± | − |
| ΔC1 mFLASH | + | − | − | + | + or ++ | ++ |
| ΔC2 mFLASH | + | − | − | + | + or ++ | ++ |
| ΔFARB mFLASH | + | + | + | + | ± | ± |
a
Various biological functions of exogenously expressed FLASH and its mutants are summarized. KB cells with Tet-On shFLASH were cultured with (+) or without (−) 1 μg/ml Dox for 3 days, and then nuclear foci of FLASH, NPAT, and coilin, the expression level of histone mRNAs, and cell cycle progression were examined as described in the legends to Fig. 1 and 3A to D (also see Fig. S1C in the supplemental material). ++, normal; +, weak but significant; ±, nearly undetectable; −, undetectable.