FIG. 7.

GATA3 upregulates Deltex1 expression. (A) Gfi1b and GATA3 expression in DN1 or DN1′ populations of cells isolated from wild-type (WT), Id1^tg, and Id1^tg/tg mice. Levels of indicated transcripts were normalized against the level of β-actin mRNA. Data shown are Gfi1b levels relative to the level in Id1^tg/tg DN1′ cells and GATA3 levels relative to the level in WT DN1 cells. They are presented as averages ± standard deviations from triplicates. (B) Bone marrow cells from 5-FU-treated WT mice were transduced with vector or GATA3-expressing retrovirus. Transduced cells were isolated based on GFP expression from the same viral constructs and used to analyze Deltex1 and Deltex4 expression. Levels of indicated mRNA, determined by using real-time RT-PCR, were normalized against the level of β-actin. Data are presented as the average expression levels ± standard deviations relative to the levels in vector-transduced cells and are calculated from triplicates. Representatives of two experiments are shown.