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. 2009 Jun 29;29(17):4864–4872. doi: 10.1128/MCB.00553-09

FIG. 5.

FIG. 5.

Effect of fasting and refeeding on SREBP mRNA and protein in livers of wild-type and 1aDf mice. mRNA expression level for SRBEP-1a (A), SREBP-1c (B), and SREBP-2 (C) in livers from wild-type and 1aDf mice from fed mice, mice fasted for 24 h, or mice fasted for 24 h and then refed a high-carbohydrate/low-fat diet for 12 h. Total RNA from livers of mice was pooled (n = 6/group) and subjected to qPCR quantification, as described in Materials and Methods. qPCR data were normalized to the L32 expression. Each value represents the amount of mRNA relative to that in the fed wild-type mice (set arbitrarily as 1). (D) Protein levels for SRBEP-1 and SREBP-2. Equal amounts from livers (n = 6/group) were pooled, nuclear proteins were extracted, and 50-μg aliquots were analyzed by SDS-PAGE and immunoblot analysis for SREBP-1 (BP-1) and SREBP-2 (BP-2). *, P < 0.001; +, P < 0.01; #, P < 0.05.