FIG. 1.

The effect of fbh1Δ and rad22Δ on Rad51 foci. (A) Examples of spread nuclei from cells grown in YES with or without CPT (10 μM)/MMS (0.03%) for 4 to 5 h. Nuclei are stained with anti-Rad51 (red) and DAPI (blue). (B) Quantification of Rad51 foci in wild-type (MCW1221), rad22Δ (MCW1285), fbh1Δ (MCW1490), and rad22Δ fbh1Δ (MCW1553) strains. Each value represents the analysis of 100 nuclei from two independent experiments. The number on top of each bar is the mean number of Rad51 foci per nucleus.