FIG. 4.
The effect of Fbh1 overexpression on repair and recombination. (A and B) Spot assays showing the sensitization to genotoxins of wild-type (MCW1221) and rad51Δ strains (MCW1088) by Fbh1 or Fbh1D485N overexpression. (C) Ade+ recombinant frequencies in a wild-type strain (MCW1262) containing the recombination substrate shown in Fig. 2A (RTS1 orientation 1) and carrying plasmids pREP41, pREP41-fbh1, and pREP41-fbh1D485N. (D) Same representation shown in panel C, except that the strain (MCW1433) contains RTS1 in orientation 2. Error bars are the standard deviations for mean values. Note that the recombination data shown here and in subsequent figures are derived from strains grown on minimal media (EMMG), whereas the data shown in Fig. 2B and C are from strains grown on YES. In most cases we observed a general reduction in the frequency of recombinants when equivalent strains were grown on EMMG compared to YES. (E) 2D gel analysis and quantification of the RFB in strain MCW1433 carrying the indicated plasmids. See the Fig. 2D legend for further details.