TABLE 1.
Schizosaccharomyces pombe strains
| Strain | Genotype | Source or reference |
|---|---|---|
| MCW1088 | h+rad51Δ::arg3+ura4-D18 leu1-32 his3-D1 arg3-D4 | Lab strain |
| MCW1221 | h+ura4-D18 leu1-32 his3-D1 arg3-D4 | Lab strain |
| MCW1230 | h+rad54Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 | Lab strain |
| MCW1262 | h−ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 1/ade6-L469 | 3 |
| MCW1285 | h+rad22Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 | 48 |
| MCW1433 | h−ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 2/ade6-L469 | 3 |
| MCW1443 | h−rqh1Δ::kanMX6 ura4-D18 his3-D1 leu1-32 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 1/ade6-L469 | 3 |
| MCW1447 | h−rqh1Δ::kanMX6 ura4-D18 his3-D1 leu1-32 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 2/ade6-L469 | 3 |
| MCW1490 | h+fbh1Δ::kanMX6 ura4-D18 leu1-32 his3-D1 arg3-D4 | 48 |
| MCW1553 | h+fbh1Δ::kanMX6 rad22Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 | 48 |
| MCW1647 | h+fbh1Δ::kanMX6 rad54Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 | This study |
| MCW1687 | h+rad22Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 1/ade6-L469 | 3 |
| MCW1688 | h+rad22Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 2/ade6-L469 | 3 |
| MCW1691 | h+rad51Δ::arg3+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 1/ade6-L469 | 3 |
| MCW1692 | h+rad51Δ::arg3+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 2/ade6-L469 | 3 |
| MCW1759 | h+fbh1D485N::kanMX6 ura4-D18 leu1-32 his3-D1 arg3-D4 | This study |
| MCW2349 | h+fbh1Δ::kanMX6 ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/lacOx40/his3+/RTS1 site A orientation 1/ade6-L469 | This study |
| MCW2351 | h+fbh1Δ::kanMX6 ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/lacOx40/his3+/RTS1 site A orientation 2/ade6-L469 | This study |
| MCW2366 | h+srs2Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/lacOx40/his3+/RTS1 site A orientation 1/ade6-L469 | This study |
| MCW2368 | h+srs2Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/lacOx40/his3+/RTS1 site A orientation 2/ade6-L469 | This study |
| MCW2406 | h+rqh1Δ::kanMX6 ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/lacOx40/his3+/RTS1 site A orientation 1/ade6-L469 | This study |
| MCW2408 | h+rqh1Δ::kanMX6 ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/lacOx40/his3+/RTS1 site A orientation 2/ade6-L469 | This study |
| MCW2648 | h+fbh1D485N::KanMX6ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 1/ade6-L469 | This study |
| MCW2873 | h+fbh1D485N::KanMX6ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 2/ade6-L469 | This study |
| FO1236 | h−ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 | Lab strain |
| FO1748 | h+srs2Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 1/ade6-L469 | This study |
| FO1750 | h+srs2Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 2/ade6-L469 | This study |
| FO1752 | h+rad22+::YFP-kanMX6 ura4-D18 leu1-32 his3-D1 | This studya |
| FO1776 | h+rad11+::GFP-kanMX6 ura4-D18 leu1-32 his3-D1 arg3-D4 | This studyb |
| FO1791 | h−ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/lacOx40/his3+/RTS1 site A orientation 1/ade6-L469 | This study |
| FO1792 | h−ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/lacOx40/his3+/RTS1 site A orientation 2/ade6-L469 | This study |
| FO1814 | h+fbh1Δ::KanMX6ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 1/ade6-L469 | This study |
| FO1816 | h+fbh1Δ::KanMX6ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 2/ade6-L469 | This study |
| FO1831 | h+fbh1Δ::arg3+rad22Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 1/ade6-L469 | This study |
| FO1833 | h+fbh1Δ::arg3+rad22Δ::ura4+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 2/ade6-L469 | This study |
| FO1860 | h+fbh1Δ::KanMX6rad51Δ::arg3+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 1/ade6-L469 | This study |
| FO1862 | h+fbh1Δ::KanMX6rad51Δ::arg3+ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M375 int::pUC8/his3+/RTS1 site A orientation 2/ade6-L469 | This study |
a
Derivative of SP220 (45).
b
Derivative of a rad11+::GFP-kanMX6 strain kindly provided by M. G. Ferreira and J. P. Cooper (CRUK, London, United Kingdom).