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. 2009 Jun 15;29(16):4552–4562. doi: 10.1128/MCB.01911-08

FIG. 5.

FIG. 5.

Effect of bir1-17 on the cellular localization of Ipl1 kinase in cells in metaphase. Asynchronous, exponentially growing cultures of wild-type BIR1 (T5241) and bir1-17 (VMY14) mutant cells expressing Ipl1-GFP and both CFP-tagged Ctf19 and Ndc80 as kinetochore markers were shifted to 37°C for 1 h, and then images were acquired. (A) Representative differential interference contrast (DIC), Ipl1-GFP (green), Ctf19/Ndc80 (red), and merged live cell images from two wild-type cells showing kinetochore-associated Ipl1-GFP (a and b) and two bir1-17 cells where no kinetochore-associated Ipl1-GFP was evident (c and d). Note, however, that both populations contained examples of both types of localization. (B) Quantitation of kinetochore-associated Ipl1-GFP in metaphase-arrested cells. n is the number of cells scored in each category.