FIG. 5.

Effect of neuron- and muscle-specific PTP1B deficiency on the AMPK pathway in EDL muscle. (A and B) α1 and α2 AMPK activities in EDL muscle of neuronal PTP1B^−/− (A) and muscle PTP1B^−/− (B) mice. (C to F) Immunoblotting of α AMPK phosphorylation, α1 and α2 AMPK, LKB1, MO25α, troponin I (slow), and β-actin protein levels in neuronal PTP1B^−/− mice. Data in panels C to E are normalized to β-actin. All experiments were carried out using 2-month-old male flox/flox (fl/fl) control, neuronal PTP1B^−/−, and muscle PTP1B^−/− mice on a chow diet. Data are expressed as means ± SEM. n = 6 to 8 for each genotype. *, P < 0.05 compared to results for WT mice.